Bacteriophage genetics an overview sciencedirect topics. A series of more than 110 articles and 6 major books followed, in which dherelle established the foundation for the later work of the phage group in molecular biology. Ocr errors may be found in this reference list extracted from the full text article. Methods and protocols is a valuable reference for experienced bacteriophage researchers as well as an easily accessible introduction for newcomers to the subject. Everyday low prices and free delivery on eligible orders. Bacteriophages are composed of proteins that encapsulate a dna or rna genome, and may have structures that are either simple. Bacteriophages viruses that infect bacteria are fascinating organisms that have played and continue to play a key role in bacterial genetics and molecular biology. In the following years, bacteriophages became important model organisms in molecular biology and genetics.
Stephen mc grath and douwe van sinderen department. Genetics and molecular biology hardback and a great selection of related books, art and collectibles available now at. Historically, they were used to define gene structure and gene regulation. Written by eminent international researchers actively involved in the disparate areas of bacteriophage research this book focuses on the current rapid developments in this exciting field. Phage can confer key phenotypes on their host, for example converting a nonpathogenic strain into a pathogen, and they. Of value to all biologists with an interest in the molecular basis of living systems, the book is a personal selection by the editor. Bacteriophages have played and continue to play a key role in bacterial genetics and molecular biology. Bacteriophage genetics modern microbial genetics wiley. The scientist who introduced the name bacteriophage was felix dherelle, who investigated these viruses for many years, leading to new fields of research, including bacteriophage therapy.
The phage genetic information then takes over the machinery of the bacterial cell by turning off the synthesis of bacterial components and redirecting the bacterial synthetic material to make more phage components figure 720. The book is written for upper level undergraduates and beginning graduate. Different altered forms of these phages are used by molecular biologists in dna cloning, where they are convenient vectors because of their infectious nature. Much of the founding work in molecular biology and structural biology was done on bacteriophages. Bacteriophages, the viruses that parasitize and kill bacteria, can be used in several different types of genetic analysis. Written by eminent international researchers actively involved in the disparate areas of bacteriophage research, this book focuses on the current rapid developments in this exciting field. Bacteriophage genetics modern genetic analysis ncbi bookshelf. Many of these tools underpin the past 50 years of molecular biology, and others. Seymour benzer used the riilocus of phage t4 to define genes by virtue of their behavior in a complementation test, and also to provide fundamental insight into the structure of genes in particular, the arrangement of mutable sites see the next section. The molecular biology of viruses is a collection of manuscripts presented at the third annual international symposium of the molecular biology of viruses, held in the university of alberta, canada on june 2730, 1966, sponsored by the faculty of medicine of the university of alberta. Oct 01, 2004 when i left the university of californiasanta cruz in 1990 and cleared out my office, i had saved a few scientific books. From the beginning, dherelle was interested in the possibility of using phages. Genetics and molecular biology inglese copertina rigida 1 lug 2007.
Phage can confer key phenotypes on their host, for example converting a nonpathogenic strain into a pathogen, and they play a key role in regulating bacterial populations in all sorts of environments. The book is a sequel to the 1983 edition and is organized into six major sections. Acm has opted to expose the complete list rather than only correct and linked references. Bacteriophages were first described and named by felix dherelle in 1917 see dherelle, felix. Bacteriophage download ebook pdf, epub, tuebl, mobi. Mutations arise in the absence of selection luria and delbruck 1943. Also the first genome to be sequenced was a bacteriophage. However, formatting rules can vary widely between applications and fields of interest or study. Since there is no preface, the editors goal can only be assumed from the contents of the book.
Bacteriophage simple english wikipedia, the free encyclopedia. Bacteriophages phages, or bacterial viruses, have been major research tools for molecular biology. Oclcs webjunction has pulled together information and resources to assist library staff as they consider how to handle coronavirus. Written in the highly successful methods in molecular biology. James dewey watson born april 6, 1928 is an american molecular biologist, geneticist and zoologist. Oct 23, 2019 before beginning the formal study of the genetics of bacteria and their viruses, it is important to understand the fundamentals of a microbial cell, chromosome, and dna structures, and the basic viral processes. These are widely used in molecular biology research and in biotechnology, as probes and markers, and in the popular method of assesing gene expression. This book apart from the numerous texts recently published in the area of bacteriophage biology is the focus on the methodology used in this field. The book is written for upper level undergraduates and beginning graduate students, particularly those who have had an introductory genetics course. Essentials of molecular genetics has been written with the objective of providing concise but complete knowledge on the abovementioned aspects of the chemical b asis of life, genetic material. Part of the methods in molecular biology book series mimb, volume 501 log in to check access.
Canchaya, marco ventura, and douwe van sinderen bacteriophages have played and continue to play a key role in bacterial genetics and molecular biology. Bacterial and bacteriophage genetics 4th edn heredity. P hage or bacteriophage is a virus that infects bacteria. The contributions of george beadle and edward tatum.
Synopsis bacteriophages viruses that infect bacteria are fascinating organisms that have played and continue to play a key role in bacterial genetics and molecular biology. In 1953, he coauthored with francis crick the academic paper proposing the double helix structure of the dna molecule. During infection, a phage attaches to a bacterium and injects its genetic material into the bacterial cytoplasm figure 719. Reliable information about the coronavirus covid19 is available from the world health organization current situation, international travel. Chapter 2 bacteriophage bioinformatics and genomics carlos a. Jim watson has written a whole book about the discovery of the. A cistrans complementation test defines a cistron, which is a gene. Molecular biology of bacteriophage t4 highlights the value of this biological system as a research and teaching tool. In 1917, dherelle published the first paper describing the phenomenon of the bacteriophage and its biological nature. Written in the highly successful methods in molecular biology series format, chapters consist of brief introductions to the subject, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and a notes section which details tips on troubleshooting and avoiding known pitfalls. All of the major topics in modern bacterial and bacteriophage genetics are presented, among them mutations and mutagenesis, genetics of t4 bacteriophage and other intemperate and temperate phages, transduction, transformation, conjugation and plasmids, recombination and repair, probability laws for prokaryote cultures, as well as applied.
Request pdf bacterial and bacteriophage genetics genetic investigations and. The focus of the book remains firmly on bacteria and will be invaluable to students studying microbiology, biotechnology, molecular biology, biochemistry, genetics and related biomedical sciences. Watson, crick and maurice wilkins were awarded the 1962 nobel prize in physiology or medicine for their discoveries concerning the molecular structure of nucleic acids and its. Bacteriophage genetics an introduction to genetic analysis. They are minute particles virions with a protein covering capsid protecting a nucleic acid genome.
Like viruses that infect eukaryotes plants, animals, and fungi there are many different phage structures and functions phages are typically made of an outer protein hull that has genetic material inside it. Bacterial genetics and bacteriophages molecular and cell. This volume is a survey of the genetics of bacteria and their viruses, and it. I read again my article in the book e dgar 1966 about the discovery of conditional lethals. This engaging book is the first full biography of dherelle, a complex figure who. This volume focuses largely on the classical protocols as described by the editors. This edition offers a greater emphasis on molecular biology and genetics than was present in either of. One was phage and the origins of molecular biology, a collection of articles honoring max delbrucks 60th birthday in 1966. In the 1940s, and onward, it was the laboratory study of phage biology that directly yielded major insights into bacterial genetics, molecular biology, and the exact manner in which viruses reproduce and spread. Role of bacteriophages in genetics and molecular biology. In his roles as director, president, and chancellor, watson led cshl to articulate its presentday mission, dedication to exploring molecular biology and genetics in order to advance the understanding and ability to diagnose and treat cancers, neurological diseases, and other causes of human suffering. Apparently this is intended as an overview of several aspects of phage biology. When i left the university of californiasanta cruz in 1990 and cleared out my office, i had saved a few scientific books.
The state of genetics in this period was described in a textbook by alfred sturtevant and george beadle, an introduction to genetics. First, two distinct phage genotypes can be crossed to measure recombination and hence map the viral genome. Before beginning the formal study of the genetics of bacteria and their viruses, it is important to understand the fundamentals of a microbial cell, chromosome, and dna structures, and the basic viral processes. All of the major topics in modern bacterial and bacteriophage genetics are presented, among them mutations and mutagenesis, genetics of t4 bacteriophage and other intemperate and temperate phages, transduction, transformation, conjugation and plasmids. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. Numerous and frequentlyupdated resource results are available from this search. Bacterial and bacteriophage genetics request pdf researchgate. Jun 24, 2019 much of what we know about genetic fine structure, prior to the advent of techniques for isolating and sequencing genes, derive form studies in bacteriophage. A, also known informally as a phage, is a virus that infects and replicates within bacteria and archaea. Biotechnology tools derived from the bacteriophagebacteria arms. The propensity of bacteriophage to recombine and the ease of measuring this recombination were crucial during the explosive period when studies of bacteriophage largely created the new field of molecular biology, as portrayed in the excellent book phage and the origins of molecular biology cairns et al. The term was derived from bacteria and the greek phagein, meaning to devour. Bacterial and bacteriophage genetics 4th edn heredity nature. Bacteriophage genetics bacteriophages, the viruses that parasitize and kill bacteria, can be used in several different types of genetic analysis.
Molecular genetics of bacteria, 5th edition microbiology. This volume is a survey of the genetics of bacteria and their viruses, and it provides students with a comprehensive introduction to this rapidly changing subject. This book reveals their compelling elegence of function and their almost inconceivable diversity. Even in the absence of other phage genes, it is active in vivo 48, but its. Dna metabolism, regulation of gene expression, phage morphogenesis, structure and function of selected proteins, hostphage interactions, and experiments in t4 molecular genetics. It starts with a chapter providing a broad overview of the topics and highlights the multifaceted nature of bacteriophage research. Also included are many important papers on structural biology and molecular genetics and papers reflecting the initial developments in dna cloning and sequencing. Bacteriophage bioinformatics and genomics bacteriophages have played and continue to play a key role in bacterial genetics and molecular biology. About this textbook genetic investigations and manipulations of bacteria and bacteriophage have made vital contributions to our basic understanding of living cells and to the development of molecular biology and biotechnology.
Working with bacteriophages and nematodes, the university of california, berkeley, molecular biologist uncovered. This book consists of 12 chapters highlighting the current rapid developments in the field of bacteriophage research. The propensity of bacteriophage to recombine and the ease of measuring this recombination were crucial during the explosive period when studies of bacteriophage largely created the new field of molecular biology, as portrayed in the excellent book phage and the. Bacteriophages viruses that infect bacteria are fascinating organisms that have played and continue to play a key role in bacterial genetics and molecular. Bacteriophage genetics modern genetic analysis ncbi. They are minute particles virions with a protein covering capsid. The term is commonly shortened to phage bacteriophages are among the most common and diverse entities in the biosphere. Genetics and molecular biology 1 by mcgrath, stephen, van sinderen, douwe isbn. In part 1, hatfull describes what bacteriophage are, some of their biological properties and how they were discovered. Phage can confer key phenotypes on their host for example, converting a nonpathogenic strain into a pathogen and they play a key role in regulating bacterial populations in all sorts of environments. In part 2, he discusses the organization of bacteriophage genomes and explains how genomic sequence comparison can provide information about viral evolution. The recombination of bacteriophage genetic markers was first observed in.
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